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Analysis of Hexabromocyclododecane (HBCDD) in Environmental Samples by Liquid Chromatography Mass Spectrometry / REBECCA CONNIFF-JENKINS

Swansea University Author: REBECCA CONNIFF-JENKINS

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Abstract

Hexabromocyclododecane (HBCDD) is a brominated flame retardant that has been identified as a priority substance within the European Water Framework Directive and must be monitored in the environment by regulatory bodies such as Natural Resources Wales. This project investigated the extraction and an...

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Published: Swansea 2022
Institution: Swansea University
Degree level: Master of Research
Degree name: MSc by Research
Supervisor: Godfrey, Ruth
URI: https://cronfa.swan.ac.uk/Record/cronfa60285
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first_indexed 2022-06-20T16:35:51Z
last_indexed 2022-06-21T03:19:11Z
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spelling 2022-06-20T17:59:47.0500900 v2 60285 2022-06-20 Analysis of Hexabromocyclododecane (HBCDD) in Environmental Samples by Liquid Chromatography Mass Spectrometry 582aff54790927ad587ce9732ed1ce0a REBECCA CONNIFF-JENKINS REBECCA CONNIFF-JENKINS true false 2022-06-20 Hexabromocyclododecane (HBCDD) is a brominated flame retardant that has been identified as a priority substance within the European Water Framework Directive and must be monitored in the environment by regulatory bodies such as Natural Resources Wales. This project investigated the extraction and analysis of the three main isomers of HBCDD in different matrices including freshwater, saline and biota, exploring different compound extraction techniques to find the optimal procedure for each matrix investigated and finding the optimal parameters for analysis. A method for analysis of aqueous saline and freshwater matrices was developed and validated involving an extraction procedure using hydrophilic-lipophilic balance (HLB) solid phase extraction (SPE) disks, elution into methanol and a x1000 concentration of the sample extract. Analysis was conducted via liquid chromatography tandem mass spectrometry with a quadrupole mass spectrometer operating in negative electrospray ionisation mode. The target limit of quantitation values for each isomer of 75 pg/L and 150 pg/L for saline and freshwater matrices respectively was achieved in both matrices, with % accuracies for both matrices ranging from 97.3 – 102.7% and every isomer in both saline and freshwater fell below the precision target of <15% RSD. Initial method development on biota matrix involved research of QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) kits with alternative dispersive SPE kits tested, and investigation into homogenisation techniques of the biota matrix. The result of this project was implementation of a fully validated method for extraction and analysis of HBCDD in saline and freshwater matrices as a routine monitoring test of HBCDD abundance in the environment. Further work will need to be conducted on the analysis of HBCDD in biota matrix. E-Thesis Swansea HBCDD, Liquid Chromatography Mass Spectrometry 8 6 2022 2022-06-08 COLLEGE NANME COLLEGE CODE Swansea University Godfrey, Ruth Master of Research MSc by Research Natural Resources Wales 2022-06-20T17:59:47.0500900 2022-06-20T17:32:31.1255354 Swansea University Medical School Medicine REBECCA CONNIFF-JENKINS 1 60285__24344__eae12e3dafad4cbc91fb277961796bc6.pdf Conniff_Jenkins_Rebecca_MSc_Research_Thesis_Final_Cronfa.pdf 2022-06-20T17:48:21.9069181 Output 3339762 application/pdf E-Thesis – open access true Copyright: The author, Rebecca Conniff-Jenkins, 2022. true eng
title Analysis of Hexabromocyclododecane (HBCDD) in Environmental Samples by Liquid Chromatography Mass Spectrometry
spellingShingle Analysis of Hexabromocyclododecane (HBCDD) in Environmental Samples by Liquid Chromatography Mass Spectrometry
REBECCA CONNIFF-JENKINS
title_short Analysis of Hexabromocyclododecane (HBCDD) in Environmental Samples by Liquid Chromatography Mass Spectrometry
title_full Analysis of Hexabromocyclododecane (HBCDD) in Environmental Samples by Liquid Chromatography Mass Spectrometry
title_fullStr Analysis of Hexabromocyclododecane (HBCDD) in Environmental Samples by Liquid Chromatography Mass Spectrometry
title_full_unstemmed Analysis of Hexabromocyclododecane (HBCDD) in Environmental Samples by Liquid Chromatography Mass Spectrometry
title_sort Analysis of Hexabromocyclododecane (HBCDD) in Environmental Samples by Liquid Chromatography Mass Spectrometry
author_id_str_mv 582aff54790927ad587ce9732ed1ce0a
author_id_fullname_str_mv 582aff54790927ad587ce9732ed1ce0a_***_REBECCA CONNIFF-JENKINS
author REBECCA CONNIFF-JENKINS
author2 REBECCA CONNIFF-JENKINS
format E-Thesis
publishDate 2022
institution Swansea University
college_str Swansea University Medical School
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hierarchy_top_title Swansea University Medical School
hierarchy_parent_id swanseauniversitymedicalschool
hierarchy_parent_title Swansea University Medical School
department_str Medicine{{{_:::_}}}Swansea University Medical School{{{_:::_}}}Medicine
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description Hexabromocyclododecane (HBCDD) is a brominated flame retardant that has been identified as a priority substance within the European Water Framework Directive and must be monitored in the environment by regulatory bodies such as Natural Resources Wales. This project investigated the extraction and analysis of the three main isomers of HBCDD in different matrices including freshwater, saline and biota, exploring different compound extraction techniques to find the optimal procedure for each matrix investigated and finding the optimal parameters for analysis. A method for analysis of aqueous saline and freshwater matrices was developed and validated involving an extraction procedure using hydrophilic-lipophilic balance (HLB) solid phase extraction (SPE) disks, elution into methanol and a x1000 concentration of the sample extract. Analysis was conducted via liquid chromatography tandem mass spectrometry with a quadrupole mass spectrometer operating in negative electrospray ionisation mode. The target limit of quantitation values for each isomer of 75 pg/L and 150 pg/L for saline and freshwater matrices respectively was achieved in both matrices, with % accuracies for both matrices ranging from 97.3 – 102.7% and every isomer in both saline and freshwater fell below the precision target of <15% RSD. Initial method development on biota matrix involved research of QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) kits with alternative dispersive SPE kits tested, and investigation into homogenisation techniques of the biota matrix. The result of this project was implementation of a fully validated method for extraction and analysis of HBCDD in saline and freshwater matrices as a routine monitoring test of HBCDD abundance in the environment. Further work will need to be conducted on the analysis of HBCDD in biota matrix.
published_date 2022-06-08T04:20:23Z
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