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Analysis of Hexabromocyclododecane (HBCDD) in Environmental Samples by Liquid Chromatography Mass Spectrometry / REBECCA CONNIFF-JENKINS
Swansea University Author: REBECCA CONNIFF-JENKINS
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Abstract
Hexabromocyclododecane (HBCDD) is a brominated flame retardant that has been identified as a priority substance within the European Water Framework Directive and must be monitored in the environment by regulatory bodies such as Natural Resources Wales. This project investigated the extraction and an...
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Swansea
2022
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Institution: | Swansea University |
Degree level: | Master of Research |
Degree name: | MSc by Research |
Supervisor: | Godfrey, Ruth |
URI: | https://cronfa.swan.ac.uk/Record/cronfa60285 |
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2022-06-20T17:59:47.0500900 v2 60285 2022-06-20 Analysis of Hexabromocyclododecane (HBCDD) in Environmental Samples by Liquid Chromatography Mass Spectrometry 582aff54790927ad587ce9732ed1ce0a REBECCA CONNIFF-JENKINS REBECCA CONNIFF-JENKINS true false 2022-06-20 Hexabromocyclododecane (HBCDD) is a brominated flame retardant that has been identified as a priority substance within the European Water Framework Directive and must be monitored in the environment by regulatory bodies such as Natural Resources Wales. This project investigated the extraction and analysis of the three main isomers of HBCDD in different matrices including freshwater, saline and biota, exploring different compound extraction techniques to find the optimal procedure for each matrix investigated and finding the optimal parameters for analysis. A method for analysis of aqueous saline and freshwater matrices was developed and validated involving an extraction procedure using hydrophilic-lipophilic balance (HLB) solid phase extraction (SPE) disks, elution into methanol and a x1000 concentration of the sample extract. Analysis was conducted via liquid chromatography tandem mass spectrometry with a quadrupole mass spectrometer operating in negative electrospray ionisation mode. The target limit of quantitation values for each isomer of 75 pg/L and 150 pg/L for saline and freshwater matrices respectively was achieved in both matrices, with % accuracies for both matrices ranging from 97.3 – 102.7% and every isomer in both saline and freshwater fell below the precision target of <15% RSD. Initial method development on biota matrix involved research of QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) kits with alternative dispersive SPE kits tested, and investigation into homogenisation techniques of the biota matrix. The result of this project was implementation of a fully validated method for extraction and analysis of HBCDD in saline and freshwater matrices as a routine monitoring test of HBCDD abundance in the environment. Further work will need to be conducted on the analysis of HBCDD in biota matrix. E-Thesis Swansea HBCDD, Liquid Chromatography Mass Spectrometry 8 6 2022 2022-06-08 COLLEGE NANME COLLEGE CODE Swansea University Godfrey, Ruth Master of Research MSc by Research Natural Resources Wales 2022-06-20T17:59:47.0500900 2022-06-20T17:32:31.1255354 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine REBECCA CONNIFF-JENKINS 1 60285__24344__eae12e3dafad4cbc91fb277961796bc6.pdf Conniff_Jenkins_Rebecca_MSc_Research_Thesis_Final_Cronfa.pdf 2022-06-20T17:48:21.9069181 Output 3339762 application/pdf E-Thesis – open access true Copyright: The author, Rebecca Conniff-Jenkins, 2022. true eng |
title |
Analysis of Hexabromocyclododecane (HBCDD) in Environmental Samples by Liquid Chromatography Mass Spectrometry |
spellingShingle |
Analysis of Hexabromocyclododecane (HBCDD) in Environmental Samples by Liquid Chromatography Mass Spectrometry REBECCA CONNIFF-JENKINS |
title_short |
Analysis of Hexabromocyclododecane (HBCDD) in Environmental Samples by Liquid Chromatography Mass Spectrometry |
title_full |
Analysis of Hexabromocyclododecane (HBCDD) in Environmental Samples by Liquid Chromatography Mass Spectrometry |
title_fullStr |
Analysis of Hexabromocyclododecane (HBCDD) in Environmental Samples by Liquid Chromatography Mass Spectrometry |
title_full_unstemmed |
Analysis of Hexabromocyclododecane (HBCDD) in Environmental Samples by Liquid Chromatography Mass Spectrometry |
title_sort |
Analysis of Hexabromocyclododecane (HBCDD) in Environmental Samples by Liquid Chromatography Mass Spectrometry |
author_id_str_mv |
582aff54790927ad587ce9732ed1ce0a |
author_id_fullname_str_mv |
582aff54790927ad587ce9732ed1ce0a_***_REBECCA CONNIFF-JENKINS |
author |
REBECCA CONNIFF-JENKINS |
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REBECCA CONNIFF-JENKINS |
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E-Thesis |
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2022 |
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Swansea University |
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Faculty of Medicine, Health and Life Sciences |
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Faculty of Medicine, Health and Life Sciences |
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Faculty of Medicine, Health and Life Sciences |
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Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine |
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description |
Hexabromocyclododecane (HBCDD) is a brominated flame retardant that has been identified as a priority substance within the European Water Framework Directive and must be monitored in the environment by regulatory bodies such as Natural Resources Wales. This project investigated the extraction and analysis of the three main isomers of HBCDD in different matrices including freshwater, saline and biota, exploring different compound extraction techniques to find the optimal procedure for each matrix investigated and finding the optimal parameters for analysis. A method for analysis of aqueous saline and freshwater matrices was developed and validated involving an extraction procedure using hydrophilic-lipophilic balance (HLB) solid phase extraction (SPE) disks, elution into methanol and a x1000 concentration of the sample extract. Analysis was conducted via liquid chromatography tandem mass spectrometry with a quadrupole mass spectrometer operating in negative electrospray ionisation mode. The target limit of quantitation values for each isomer of 75 pg/L and 150 pg/L for saline and freshwater matrices respectively was achieved in both matrices, with % accuracies for both matrices ranging from 97.3 – 102.7% and every isomer in both saline and freshwater fell below the precision target of <15% RSD. Initial method development on biota matrix involved research of QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) kits with alternative dispersive SPE kits tested, and investigation into homogenisation techniques of the biota matrix. The result of this project was implementation of a fully validated method for extraction and analysis of HBCDD in saline and freshwater matrices as a routine monitoring test of HBCDD abundance in the environment. Further work will need to be conducted on the analysis of HBCDD in biota matrix. |
published_date |
2022-06-08T04:18:16Z |
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10.997956 |